Biohazardous Waste Management

Definition: Waste which has the perceived or actual presence of pathogenic agents. "RED BAG" waste requires decontamination and/ or treatment to prevent occupational or environmental exposure.

Examples of Infectious Waste includes:

  • microbiological cultures
  • blood and blood products
  • pathological waste (human/animal organs, tissues, bodily fluids, etc.)
  • Sharps (needles, syringes, razor blades, Pasteur pipettes,etc.)
  • Animals, body parts, and bedding
  • Chemotherapeutic agents and waste
  • Cytological drugs
  • Any solid waste contaminated with Infectious material

General Instructions

Pathological/anatomical/chemotherapeutic waste must be disposed of in red biohazard waste bags. Red bag waste is received by EH&S in room WG-01 TRB, the fourth Wednesday of every month from 2:00-2:30 p.m. More frequent transfers (if necessary) may be arranged by appointment.

  • Animal carcasses and/or parts must be bagged and transferred to the Research Resource Facility (RRF)
  • Liquids must not be placed in red bags, but must be decontaminated and properly disposed of (see above)
  • Red bag waste must be stored in a leak proof container with the biohazard label affixed
  • Empty brown bottle chemical containers

Autoclaving/ Steam Sterilization (Orange Bags only)

General Instructions

Potentially infectious/ biohazards materials (except for animal carcasses, body parts, and/or tissues) may be autoclaved, then disposed of as regulated waste

  1. Only use Orange color autoclave bags
  2. Fill the autoclave no more than 3/4 full
  3. Do not close bag tightly. Allow enough room at the opening for steam to enter and escape the bag
  4. Always use autoclave indicator tape
  5. Always place bags in an autoclave tray
  6. Never place sharps in the autoclave (Sharps must be taken to WG-01 The Research Building, the fourth Wednesday of every month from 2:00-2:30pm for disposal)
  7. Do not autoclave animal carcasses or body parts (these bags must be bagged and returned to the RRF)
  8. Do not autoclave pathological/anatomical waste (These materials must be placed in a red bag- See Red Bag Waste section above)
  9. To sterilize regulated waste materials, run the autoclave cycle for at least 45 minutes at 121C, with a drying cycle time of 15 minutes
  10. Promptly remiove the bag following the completion
  11. Inspect for complete sterilization, leakage, or protruding objects (i.e. pipettes)
  12. Tightly seal the bag closed
  13. Place the Orange autoclave bag inside the gray trash container lined with a clear plastic bag

Note: Do not leave unautoclaved waste in sterilization area